中文名稱(chēng) :磷酸二脂酶
其他中文名稱(chēng) :磷酸二酯酶Ⅱ;磷酸二酯酶
英文名稱(chēng) : Phosphodiesterase II from bovine spleen
其他英文名稱(chēng) : 3′-Exonuclease;Spleen exonuclease;Spleen phosphodiesterase
產(chǎn)品規(guī)格 : BR,10u/mg
C A S :9068-54-6
級(jí) 別 :BR
Activity :≥10u/mg
活力定義 :One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate
產(chǎn)品描述 :Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique
性 狀 :粉末
用 途 :生化研究。
保 存 :-20℃